#### 8.2.58.54 tblish.dataset.Puromycin ¶

Static Method: `out =` Puromycin `()` ¶

  Reaction Velocity of an Enzymatic Reaction

  #### Description ¶

  Reaction velocity versus substrate concentration in an enzymatic reaction involving untreated cells or cells treated with Puromycin.

  #### Format ¶

  `state`

    Whether the cell was treated.

  `conc`

    Substrate concentrations (ppm).

  `rate`

    Instantaneous reaction rates (counts/min/min).

  #### Details ¶

  Data on the velocity of an enzymatic reaction were obtained by Treloar (1974). The number of counts per minute of radioactive product from the reaction was measured as a function of substrate concentration in parts per million (ppm) and from these counts the initial rate (or velocity) of the reaction was calculated (counts/min/min). The experiment was conducted once with the enzyme treated with Puromycin, and once with the enzyme untreated.

  #### Source ¶

  The data are given in Box & Jenkins (1976). Obtained from the Time Series Data Library at http://www-personal.buseco.monash.edu.au/~hyndman/TSDL/.

  #### References ¶

  Bates, D.M. and Watts, D.G. (1988). Nonlinear Regression Analysis and Its Applications. New York: Wiley. Appendix A1.3.

  Treloar, M. A. (1974). Effects of Puromycin on Galactosyltransferase in Golgi Membranes. M.Sc. Thesis, U. of Toronto.

  #### Examples ¶

      t = tblish.dataset.Puromycin;

      # TODO: Port example to Octave